First Report of Head Blight of Wheat Caused by <i>Fusarium vorosii</i> in Serbia

HomePlant DiseaseVol. 106, No. 2First Report of Head Blight Wheat Caused by Fusarium vorosii in Serbia PreviousNext DISEASE NOTE OPENOpen Access licenseFirst SerbiaA. Obradović, J. Stepanović, V. Krnjaja, A. Bulajić, G. Stanković, M. Stevanović, and S. StankovićA. Obradović†Corresponding author: Obradović; E-mail Address: [email protected]://orcid.org/0000-0001-8215-7690Maize Research Institute, Zemun Polje, 11185 Belgrade, SerbiaSearch for more papers this author, StepanovićInstitute Pesticides Environmental Protection, 11080 KrnjajaInstitute Animal Husbandry, Bulajićhttps://orcid.org/0000-0001-7680-9664Faculty Agriculture, University StankovićMaize Slobodana Bajića 1, StevanovićMaize author AffiliationsAuthors Affiliations Obradović1 † Stepanović2 Krnjaja3 Bulajić4 Stanković5 Stevanović5 1Maize 2Institute 3Institute 4Faculty 5Maize Published Online:31 Jan 2022https://doi.org/10.1094/PDIS-04-21-0715-PDNAboutSectionsPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat The cosmopolitan species graminearum Schwabe directly reduces yield, as well grain quality cereals, due its ability synthesize mycotoxins. Previously it was considered be one occurring on all continents. However, phylogenetic analysis employing the GCPSR method (genealogical concordance recognition) revealed existence 15 within what is now recognized complex (FGSC) (Sarver et al. 2011). During 1996–2008, a collection FGSC isolates established at Maize originating from wheat (5), maize (3), barley (2) were selected further study. Morphological features including appearance colonies macroconidia (average size 38.5 53.1 × 4.6 5.4 µm, n = 50) 10 potato dextrose agar (PDA) consistent with descriptions F. (Leslie Summerell 2006; O’Donnell 2004). Total DNA isolated mycelium removed 7-day-old single-spore grown PDA using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Further identification based amplification sequencing elongation factor TEF-1α, histone H3, β-tubulin both directions, primers ef1/ef2, H3-1a/H3-1b, T1/T22, respectively (Jacobs 2010). sequences deposited GenBank under accession numbers MF974399–MF974408 (TEF-1α), MG063783–MG063792 (β-tubulin), MF999139–MF999148 (histone H3). Sequence performed BLAST while genetic similarity calculated MEGA 6.0 software. Isolate 1339 (collected locality Kikinda 2006) shared 100% nucleotide identity TEF-1α (DQ459745), H3 (DQ459728), (DQ459643) isolate NRRL37605 (Starkey 2007). remaining nine identified they 99 NRRL 28439 (O’Donnell Pathogenicity tested artificial inoculations spikes during flowering (Mesterházy 1999). Thirty classes inoculated each isolate, three replicates. Inoculum prepared PDA, 30 ml conidial suspension (1 105 conidia/ml) used. Control plants sterile water. Three weeks after inoculation, typical head blight symptoms visible plants, which successfully reisolated. remained symptomless. Disease severity estimated 1 7 scale (Blandino 2012). Average pathogenicity 1.9, 2.4–5.1 isolates. Toxin production determined gas chromatography-tandem mass spectrometry. Kernels ground presence deoxynivalenol (DON) acetyl derivatives 3ADON, 15ADON, NIV. possessed 15ADON chemotype, eight isolates, only 3ADON chemotype. To date, has been detected Hungary (Tóth 2005) Korea barley, corn, rice (Lee 2016). This first report Serbia, great importance, because indicates spread toxigenic species. studies should focused determining distribution, aggressiveness, toxicological profile vorosii.The author(s) declare no conflict interest.References:Blandino, M., 2012. Field Crops Res. 133:139. https://doi.org/10.1016/j.fcr.2012.04.004 Crossref, ISI, Google ScholarJacobs, A., 2010. Fungal Biol. 114:515. https://doi.org/10.1016/j.funbio.2010.03.013 ScholarLee, T., 2016. Pathol. 32:407. https://doi.org/10.5423/PPJ.OA.05.2016.0123 ScholarLeslie, F., Summerell, B. eds. 2006. Laboratory Manual. Blackwell Publishing, Ames, IA. https://doi.org/10.1002/9780470278376 ScholarMesterházy, 1999. Breed. 118:97. https://doi.org/10.1046/j.1439-0523.1999.118002097.x ScholarO’Donnell, K., 2004. Genet. 41:600. https://doi.org/10.1016/j.fgb.2004.03.003 ScholarSarver, J., 2011. 48:1096. https://doi.org/10.1016/j.fgb.2011.09.002 ScholarStarkey, D. E., 2007. 44:1191. https://doi.org/10.1016/j.fgb.2007.03.001 ScholarTóth, B., 2005. Eur. 113:35. https://doi.org/10.1007/s10658-005-0296-y ScholarThe interest.DetailsFiguresLiterature CitedRelated Vol. 2 February 2022SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionWater-soaked lesions bougainvillea caused Robbsia andropogonis (O. Morales-Galván al.). Photo credit: L. Flores-López. Soybean leaf showing crinkling downward curling, characteristic infection soybean mosaic virus (SMV) (S. van Bentum Bentum. Metrics Downloaded 644 times Article History Issue Date: 28 Feb 2022Published: 31 2022First Look: 8 Jul 2021Accepted: 17 Jun 2021 Page: 758 Information© 2022 American Phytopathological SocietyKeywordsfungifield cropscereals grainspathogen detectionFusarium complexThe interest.PDF download